Related Experiment Video
Updated: Aug 3, 2026

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays
Published on: March 14, 2011
Abstract:
Two populations of human blood lymphocytes--one forming rosettes with sheep erythrocytes, the other non-rosetting--are cytolytic in vitro for several long term cultured tumour-derived cell lines. A particular breast cancer-derived target cell (MDA-157) is only killed by the non-rosetting effector. Rosetting cells from normal donors infected or immunized with influenza virus augment cytolytic activity on MDA-157 targets by non-rosetting effector cells. Similar augmenting activity can be induced by incubating the rosetting population with sources of immune (gamma), but not leucocyte (alpha) interferon in vitro. This augmentation of cytolytic activity does not require compatibility at the major histocompatibility locus between the augmenting and effector populations.
More Related Videos
Related Concept Videos
Cells of the Innate Immune Response
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Immune Surveillance by NK Cells and Phagocytes
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Cytotoxic T Cells-mediated Immune Response
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immune Response Against Viral Pathogens
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...

