A standardized differential stain for hematology
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Updated: Aug 12, 2026

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
Published on: August 9, 2019
Abstract:
A stain composed of purified Azure B and Eosin Y has been developed for use in conjunction with an automated stainer. This system provides the consistency in chromatic properties required for automated blood-cell analysis by pattern recognition systems. The stain represents a marked improvement in stability over those presently used with differential analyzers, but it is also an important advance toward a standardized stain for general hematological use. The purity of the component dyes, the stability of the final stain and the dependence of staining intensity on the stain/buffer ratio and dye concentration are discussed.
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