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Local passive transfer of delayed-type hypersensitivity in the mouse
Journal of Immunology (Baltimore, Md. : 1950)
|July 1, 1982
Summary
Researchers developed a new method for transferring delayed-type hypersensitivity (DTH) responses in mice. This technique uses peritoneal exudate cells and offers a sensitive tool for studying DTH mechanisms and immune modulation.
Area of Science:
- Immunology
- Cellular immunology
- Hypersensitivity
Background:
- Delayed-type hypersensitivity (DTH) is a critical immune response.
- Understanding DTH mechanisms requires effective experimental models.
- Local passive transfer systems are valuable for studying cell-mediated immunity.
Purpose of the Study:
- To establish and characterize a novel method for local passive transfer of DTH in mice.
- To utilize this system for identifying DTH-mediating cells and their interactions.
- To assess the system's utility as a sensitive indicator of immunomodulatory activity.
Main Methods:
- Induction of peritoneal exudates (PE) cells in mice post-immunization.
- Intradermal transfer of PE cells and antigen into naive mouse footpads.
- Measurement of antigen-specific footpad swelling as an indicator of DTH.
- Assessment of T cell dependence using anti-theta serum treatment.
Main Results:
- Demonstrated successful local passive transfer of DTH using PE cells in mice.
- Observed significant antigen-specific footpad swelling (20-80%) with PE cells, superior to spleen or lymph node cells.
- Response magnitude correlated with cell and antigen dosage.
- Confirmed T cell dependence of the DTH transfer.
Conclusions:
- The developed murine local transfer system is superior for DTH studies.
- This model provides a potent tool for identifying DTH-mediating cells and their interactions.
- The system serves as a sensitive assay for evaluating immunomodulatory effects in cell-free supernatants.