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The ethanol gelation test and thrombosis.

H C Godal, F Brosstad, P Kierulf

Bibliotheca Haematologica

|January 1, 1977

Summary

Rapid paracoagulation tests, like the ethanol gelation test (EGT), are crucial for detecting intravascular fibrin formation in emergency situations. These tests offer a quick method for identifying fibrin, a key component in thrombus formation and thrombotic disorders.

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Area of Science:

Hematology
Clinical Chemistry

Background:

Intravascular fibrin formation is central to thrombus development and can occur due to coagulation activation in circulating blood.
Fibrin incorporation in thrombi can be detected using 125I-labelled fibrinogen.
Soluble fibrin indicates thrombophilic states, thromboembolic complications, and disseminated intravascular coagulation (DIC).

Purpose of the Study:

To review the current status and utility of rapid diagnostic tests for intravascular fibrin formation.
To highlight the importance of paracoagulation tests, particularly the ethanol gelation test (EGT), for emergency settings.

Main Methods:

Detection of incorporated fibrin using 125I-labelled fibrinogen.
Quantitation of soluble fibrin via N-terminal amino acid analysis, chromatography, and paracoagulation tests (ethanol, protamine sulfate).
Indirect assay of fibrinogen-to-fibrin transformation by measuring released fibrinopeptide A using immunological techniques.

Main Results:

Paracoagulation tests, including the ethanol gelation test (EGT), are suitable for routine and emergency use due to their speed and simplicity.
Various techniques exist for detecting and quantifying fibrin and its precursors, but practical limitations exist for immediate clinical application.

Conclusions:

Rapid and simple tests are essential for diagnosing conditions involving intravascular fibrin formation, especially in emergency medical scenarios.
The ethanol gelation test (EGT) is presented as a valuable tool for the rapid detection of intravascular fibrin in clinical practice.

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