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Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
13:08

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Published on: September 9, 2012

[Factor VIII activity and factor VIII-associated antigen in healthy newborns and in newborns with stressing perinatal

B Maak, J Frenzel

    Folia Haematologica (Leipzig, Germany : 1928)
    |January 1, 1982
    PubMed

    Insights

    Healthy newborns have normal coagulation factor VIII activity (VIII:C). However, newborns and "risk children" show elevated factor VIII-associated antigen (VIII:AGN) levels, suggesting stress impacts coagulation factors.

    Area of Science:

    • Hematology
    • Pediatric Coagulation
    • Neonatal Physiology

    Context:

    • Investigating coagulation factor levels in newborns is crucial for understanding neonatal hemostasis.
    • Perinatal factors can influence neonatal coagulation profiles.
    • Establishing baseline levels for factor VIII coagulation activity (VIII:C) and factor VIII-associated antigen (VIII:AGN) in healthy and at-risk newborns is important.

    Purpose:

    • To compare factor VIII coagulation activity (VIII:C) and factor VIII-associated antigen (VIII:AGN) levels in healthy newborns versus newborns with perinatal risk factors.
    • To analyze the elimination rate of VIII:AGN in these two groups.
    • To explore the potential causes for observed differences, such as stress and thrombin effects.

    Summary:

    • Factor VIII coagulation activity (VIII:C) levels in healthy newborns are comparable to adult levels.
    • Both healthy newborns and newborns with perinatal risk factors (
    • risk children
    • ) exhibit significantly increased VIII:AGN concentrations on the first day of life.
    • VIII:AGN is eliminated more slowly in risk children compared to healthy newborns.
    • Increased VIII:AGN is attributed to birth-related stress, and the VIII:C/VIII:AGN discrepancy may result from a thrombin effect.

    Impact:

    • These findings provide insights into neonatal coagulation dynamics and the influence of perinatal stress.
    • Understanding these differences can aid in the clinical assessment of newborns, particularly those with risk factors.
    • The study highlights the distinct behavior of VIII:C and VIII:AGN under specific neonatal conditions.

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