Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

EMOSS: an epiillumination microscope objective slit-scan flow system.

L A Weller, L L Wheeless

    Cytometry
    |July 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Chromosome 9 monosomy by fluorescence in situ hybridization of bladder irrigation specimens is predictive of tumor recurrence.

    The Journal of urology·1999
    Same author

    Rational-emotive behavior therapy and the formation of stimulus equivalence classes.

    Journal of clinical psychology·1998
    Same author

    DNA cytometry and chromosome 9 aberrations by fluorescence in situ hybridization of irrigation specimens from bladder cancer patients.

    Urology·1998
    Same author

    Biomarker study of primary nonmetastatic versus metastatic invasive bladder cancer. National Cancer Institute Bladder Tumor Marker Network.

    Clinical cancer research : an official journal of the American Association for Cancer Research·1998
    Same author

    Gender differences in the sexual rating of words.

    Journal of sex & marital therapy·1998
    Same author

    Loss of the CDKN2A/p16 locus detected in bladder irrigation specimens by fluorescence in situ hybridization.

    The Journal of urology·1997
    Same journal

    Measurement of the distribution of red blood cell deformability using an automated rheoscope.

    Cytometry·2002
    Same journal

    Flow cytometric-based isolation of nucleated erythroid cells during maturation: an approach to cell surface antigen studies.

    Cytometry·2002
    Same journal

    Flow cytometric immunophenotyping test for staging/monitoring neuroblastoma patients.

    Cytometry·2002
    Same journal

    Determination of bronchoalveolar lavage leukocyte populations by flow cytometry in patients investigated for respiratory disease.

    Cytometry·2002
    Same journal

    Multicenter clinical experience with flow cytometric method for fetomaternal hemorrhage detection.

    Cytometry·2002
    Same journal

    Separation of live cells in different phases of the cell cycle for gene expression analysis.

    Cytometry·2002
    See all related articles

    A novel epiillumination microscope objective slit-scan flow system uses hydrodynamic focusing for cellular fluorescence analysis. This system enables low-resolution imaging of cell fluorescence and light scattering, potentially avoiding the need for 3D systems.

    Area of Science:

    • Biophotonics
    • Cellular Imaging
    • Flow Cytometry

    Background:

    • Traditional flow cytometry systems may require complex 3D analysis for cellular imaging.
    • Hydrodynamic focusing is a technique used to align particles or cells in a fluid stream.

    Purpose of the Study:

    • To develop and characterize a novel epiillumination microscope objective slit-scan flow system.
    • To enable low-resolution analysis of cellular fluorescence using hydrodynamic focusing and slit-scanning.
    • To investigate the potential for preferential cell orientation to simplify imaging.

    Main Methods:

    • Fabrication of an epiillumination microscope objective slit-scan flow system.
    • Utilizing two-dimensional slit scanning with hydrodynamic sample stream focusing.

    Related Experiment Videos

  • Collecting and imaging cellular fluorescence using a high numerical aperture optical system and photomultiplier tubes.

    • Main Results:

    • Demonstrated a stabilized flow plane through hydrodynamic focusing for low-resolution (4 micron) analysis.
    • Generated two orthogonal slit-scan contours as cells traversed the excitation region.
    • Successfully collected and detected cellular fluorescence.

    Conclusions:

    • The developed system facilitates low-resolution cellular fluorescence analysis.
    • Preferential cell orientation in the stabilized flow plane may preclude the need for 3D systems.
    • The system is adaptable for various fluorescent stains, markers, and light scattering studies.