Related Experiment Video
Updated: Aug 14, 2026
12:15
Generation of Monoclonal Antibodies Against Natural Products
Published on: April 6, 2019
A modified ELISA technique for anti-hapten antibodies
Journal of Immunological Methods
|August 27, 1982
Abstract:
Polystyrene tubes pretreated with glutaraldehyde in phosphate buffer were used in a solid-phase enzyme-linked immunoassary (ELISA). The hapten o-dinitrocarboxyphenol (o-DNCP) was used as coat. The binding of homologous rabbit antibodies was measured with phosphatase-labelled staphylococcal protein A (SPA-PH). This method is both very sensitive (serum dilutions greater than 2 x 10(-6) were still positive) and highly reproducible. Anti-DNP rat IgE was detected with o-DNCP hapten as a coat using avidin/biotin as a marker.
Related Concept Videos
01:31Hybridoma Technology
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
01:33Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.