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Serum-plasma separator in amylase assay.
The American Journal of Medical Technology
|September 1, 1982
Summary
This study introduces a modified Amylochrome method using Sure-Sep II Serum-Plasma Separators for faster amylase activity testing. The new method shows excellent correlation with the reference, ensuring accurate results without interference.
Area of Science:
- Clinical Chemistry
- Biochemical Assays
- Laboratory Diagnostics
Background:
- The Amylochrome method is a standard for determining amylase activity.
- Efficient separation of supernatant and precipitant is crucial for accurate results.
- Centrifugation time can impact the speed of diagnostic testing.
Purpose of the Study:
- To evaluate a modified Amylochrome method incorporating Sure-Sep II Serum-Plasma Separators.
- To assess the impact of these separators on amylase activity measurements.
- To determine if the modification improves efficiency without compromising accuracy.
Main Methods:
- A modified Amylochrome procedure utilizing Sure-Sep II Serum-Plasma Separators was developed.
- Patient sera (n=30) and control sera (n=20) were analyzed in duplicate.
- Results from the modified method were compared against the reference Amylochrome method.
Main Results:
- The Sure-Sep II separators did not interfere with amylase activity.
- An excellent correlation (r = 0.99) was observed between the reference and modified methods.
- Significant time savings were achieved during the separation process.
Conclusions:
- The modified Amylochrome method with Sure-Sep II Serum-Plasma Separators is a valid and reliable approach.
- This modification offers a faster and more efficient way to determine amylase activity.
- The technique is suitable for routine clinical laboratory use.