A transcannula method for subcortical HRP gel implants: inferior olive afferents in the rat

A technique for using horseradish peroxidase in a polyacrylamide gel form for subcortical implants is described. In order to prevent uptake along the injection tract, the solid HRP gel pellet is delivered through a pre-implanted cannula to the target site. The appreciable advantages of using the HRP-transcannula technique are discussed in conjunction with a description of the afferents to the inferior olivary complex in the rat. The principal sources of afferents were the ipsilateral prerubral parafascicular nucleus, contralateral lateral cerebellar nucleus and dorsal column nuclei.