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Early sequential development in avian gonads. An ultrastructural study using selective glycogen labeling in the germ
Reproduction, Nutrition, Developpement
|January 1, 1981
Summary
This study details early chick embryo gonad development, revealing the origin of the gonadal medulla from germinal epithelium and mesenchyme. It also describes primordial germ cell migration and interactions with somatic cells.
Area of Science:
- Developmental Biology
- Cell Biology
- Histology
Background:
- The origin of the avian gonadal medulla is a debated topic in developmental biology.
- Understanding early gonad development is crucial for avian embryology research.
Purpose of the Study:
- To investigate the early development of chick embryo gonads using advanced microscopy techniques.
- To elucidate the origin of the avian gonadal medulla and the behavior of primordial germ cells (PGCs).
Main Methods:
- Light and transmission electron microscopy were employed.
- Cytochemical detection of glycogen in PGCs was utilized for cell tracking.
- Observations spanned from day 2 to day 5 of embryonic development (stages 14-27).
Main Results:
- The primitive gonadal medulla originates from the germinal epithelium and epithelioid cords, with contributions from intermediate mesoderm mesenchyme.
- Mesenchymal condensation involves cell aggregation via dense plaques and extracellular matrix deposition.
- Migrating PGCs interact with fibroblast-like somatic cells through dense plaques and exhibit characteristic migratory cell morphology.
Conclusions:
- The study provides insights into the controversial origin of the avian gonadal medulla.
- The mesodermal tissue environment, with its extracellular compartment and basal lamina discontinuities, facilitates PGC invasion and migration.