Anti-trypanosomal factor in the haemolymph of Glossina

The motility of cultured procyclic forms of Trypanosoma brucei brucei ANTat serodeme derived from EATRO 1125 was greatly reduced when incubated in vitro with the haemolymph of Glossina morsitans morsitans at dilutions as low as 1:512 after incubation periods of 1-2 h at 27 degrees C. This effect was demonstrated in the haemolymph of male and female, teneral and non-teneral G.m. morsitans but was abolished by heat inactivation. A significant reduction in the motility of cultured forms of Trypanosoma (S.) dionisii was also observed when incubated in G.m. morsitans haemolymph but little effect was seen on culture forms of Crithidia fasciculata and Leishmania hertigi. An anti-trypanosomal factor was also demonstrated in the haemolymph of Glossina austeni, G. palpalis gambiensis and G. tachinoides.