Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phosphonate utilization by bacteria.

A M Cook, C G Daughton, M Alexander

    Journal of Bacteriology
    |January 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Researchers discovered bacteria that can use 2-aminoethylphosphonic acid (AEP) as a sole source of carbon, nitrogen, and phosphorus. This study marks the first demonstration of phosphonoalkyl moiety utilization as a carbon source.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    COVID-19: Seroprevalence and Vaccine Responses in UK Dental Care Professionals.

    Journal of dental research·2021
    Same author

    Validation of a combined ELISA to detect IgG, IgA and IgM antibody responses to SARS-CoV-2 in mild or moderate non-hospitalised patients.

    Journal of immunological methods·2021
    Same author

    Two-dimensional type-II Dirac fermions in layered oxides.

    Nature communications·2018
    Same author

    Direct observation of orbital hybridisation in a cuprate superconductor.

    Nature communications·2018
    Same author

    A trans-Atlantic examination of haddock Melanogrammus aeglefinus food habits.

    Journal of fish biology·2016
    Same author

    Lunar atmosphere. How surface composition and meteoroid impacts mediate sodium and potassium in the lunar exosphere.

    Science (New York, N.Y.)·2015
    Same journal

    Development of a gene-editing strategy to overcome genetic intractability in <i>Lactobacillus johnsonii</i>.

    Journal of bacteriology·2026
    Same journal

    Bactofilins are essential spatial organizers of peptidoglycan insertion in the Lyme disease spirochete <i>Borrelia burgdorferi</i>.

    Journal of bacteriology·2026
    Same journal

    DNA damage-associated vesicle production in <i>Stenotrophomonas maltophilia</i> is mediated by the maltocin endolysin.

    Journal of bacteriology·2026
    Same journal

    Characterization of <i>Helicobacter pylori</i> aggregation reveals a requirement for both AlpA and AlpB.

    Journal of bacteriology·2026
    Same journal

    Cross-regulation of amino acid synthesis and anaerobic electron transfer by MetR-mediated methionine signaling.

    Journal of bacteriology·2026
    Same journal

    YpeB stability affects germination possibly through delaying SleB activity in <i>Bacillus subtilis</i>.

    Journal of bacteriology·2026
    See all related articles

    Area of Science:

    • Microbiology
    • Environmental Science
    • Biochemistry

    Background:

    • Phosphonates are organophosphorus compounds with diverse environmental and biological roles.
    • Bacteria utilize various compounds as nutrient sources, but the use of phosphonates as a sole carbon source is less understood.

    Purpose of the Study:

    • To isolate and characterize bacteria capable of utilizing alkylphosphonates as nutrient sources.
    • To investigate the microbial utilization of 2-aminoethylphosphonic acid (AEP) as a sole source of carbon, nitrogen, and phosphorus.

    Main Methods:

    • Isolation of bacteria from soil and sewage samples using phosphonates as selective media.
    • Growth studies with identified bacterial isolates using AEP and other phosphonates.
    • Biochemical analysis of AEP metabolism in Pseudomonas putida, including enzyme assays and product identification.

    Related Experiment Videos

    Main Results:

    • Several bacterial isolates were obtained that could utilize alkylphosphonates as phosphorus sources.
    • Four isolates, including Pseudomonas putida, demonstrated the ability to use AEP as a sole source of carbon, nitrogen, and phosphorus.
    • Pseudomonas putida released orthophosphate and ammonium from AEP and showed an inducible enzyme system for AEP breakdown, producing acetaldehyde.

    Conclusions:

    • This study demonstrates for the first time the utilization of a phosphonoalkyl moiety as a sole carbon source by bacteria.
    • The findings reveal a novel metabolic pathway for AEP degradation in Pseudomonas putida, involving an inducible enzyme system.