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Quantitation of 25-hydroxycholecalciferol in human serum by high-pressure liquid chromatography
The Journal of Laboratory and Clinical Medicine
|January 1, 1978
Abstract:
An HPLC method for assaying 25(OH)D3 in extracts of human serum is described. The method involves extraction of serum with methanol-dichloromethane and chromatography on a column of Sephadex LH-20 prior to HPLC with quantitation by UV absorbance. Comparison with a CBP assay for 25(OH)D3 showed no difference between the two methods.