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Immunochemical determinant of Candida parapsilosis
Carbohydrate Research
|June 16, 1983
Summary
Candida parapsilosis cell-wall D-mannan oligosaccharides were analyzed. Hexasaccharide M6 showed strong inhibitory activity, suggesting it may be immunodominant and responsible for C. parapsilosis mannan specificity.
Area of Science:
- Carbohydrate chemistry
- Immunochemistry
- Microbiology
Background:
- Candida parapsilosis is an opportunistic fungal pathogen.
- Cell-wall mannans are key components of Candida species and are involved in host-pathogen interactions.
- Understanding the structure of C. parapsilosis mannan is crucial for developing diagnostic and therapeutic strategies.
Purpose of the Study:
- To elucidate the structures of oligosaccharides derived from C. parapsilosis cell-wall D-mannan.
- To evaluate the immunoinhibitory activity of these oligosaccharides.
Main Methods:
- Alkali extraction and copper complex purification of D-mannan.
- Acetolysis and Bio-Gel P-2 filtration for oligosaccharide isolation.
- 1H-n.m.r. spectroscopy, methylation analysis, and partial acid hydrolysis for structural determination.
- Inhibition assays using anti-C. parapsilosis serum and homologous D-mannan.
Main Results:
- Six oligosaccharides were obtained from C. parapsilosis D-mannan.
- The structures of the penta- and hexasaccharide (M5 and M6) were determined.
- Both M5 and M6 demonstrated significant inhibitory activity against anti-C. parapsilosis serum.
- M6 exhibited stronger inhibitory activity than M5.
Conclusions:
- The hexasaccharide M6 is a potent inhibitor of the precipitin reaction.
- M6 may be the immunodominant epitope of C. parapsilosis mannan.
- M6 might be responsible for the specificity of C. parapsilosis mannan in immunological assays.