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Related Experiment Videos

Trypanosoma cruzi: surface antigenic determinants.

W M Tamashiro, D Repka, J K Sakurada

    Zeitschrift Fur Parasitenkunde (Berlin, Germany)
    |January 1, 1983
    PubMed
    Summary

    A novel parasite fraction (FAd) was isolated and characterized, showing inhibitory properties against specific agglutination reactions. This fraction

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    Area of Science:

    • Parasitology
    • Immunochemistry
    • Biochemistry

    Background:

    • Epimastigote forms of parasites can trigger specific immune responses.
    • Characterizing parasite antigens is crucial for understanding host-parasite interactions and developing diagnostics or therapeutics.

    Purpose of the Study:

    • To isolate and characterize a specific fraction (FAd) from parasite lysates.
    • To investigate the antigenic properties and inhibitory capabilities of FAd.
    • To determine the nature of the antigenic determinants within FAd.

    Main Methods:

    • Extraction of lyophilized epimastigote lysates with buffered saline.
    • Detection of parasite proteinase activity.
    • Precipitation of FAd components using Concanavalin A, methylated albumins, and HCl.
    • Electrophoretic analysis (agarose and polyacrylamide gel electrophoresis with SDS).
    • Immunization of rabbits and characterization of induced antibodies.
    • Inhibition assays using FAd and parasite proteinase.

    Main Results:

    • A fraction (FAd) was obtained that inhibited specific agglutination reactions.
    • FAd contained four glycopeptide components and exhibited antigenic determinants resistant to heat and pronase but sensitive to periodate, suggesting polysaccharide characteristics.
    • Antibodies generated against FAd reacted with various parasite forms and inhibited agglutination reactions, while purified proteinase did not.

    Conclusions:

    • The study identified a parasite fraction (FAd) with significant antigenic properties.
    • FAd's characteristics suggest it contains polysaccharide-based antigenic determinants.
    • FAd and its induced antibodies show potential for diagnostic applications in parasitic infections.

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