Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Modified method of staining semi-thin sections with hematoxylin and eosin].

N S Migalkin, Iu M Ir'ianov

    Arkhiv Patologii
    |January 1, 1983
    PubMed
    Summary

    This study presents an improved hematoxylin and eosin staining method for semi-thin sections. The technique effectively removes embedding resins and enhances nuclear and cytoplasmic contrast for better visualization.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Morphological characteristics of paravertebral muscles in patients with scoliosis caused by primaryprogressive muscular dystrophies].

    Arkhiv patologii·2019
    Same author

    [Fracture healing under intramedullary insertion of wires with hydroxyapatite coating].

    Vestnik Rossiiskoi akademii meditsinskikh nauk·2015
    Same author

    [Morphological characteristics of reparative osteogenesis under the conditions of transosseous osteosynthesis and intramedullary introduction of hydroxyapatite-coated wires].

    Morfologiia (Saint Petersburg, Russia)·2015
    Same author

    [Bone defect replacement under conditions of transosseous osteosynthesis and titanium nickelide implant application].

    Morfologiia (Saint Petersburg, Russia)·2012
    Same author

    [Computer karyometry of the thyroid thyrocytes in the conditions of modeling acute radiation injury in animals].

    Radiatsionnaia biologiia, radioecologiia·2008
    Same author

    [Repair osteogenesis during limb elongation using transosseous distraction osteosynthesis].

    Morfologiia (Saint Petersburg, Russia)·2003

    Area of Science:

    • Histology
    • Microscopy techniques
    • Staining protocols

    Context:

    • Semi-thin section preparation for microscopy often involves embedding resins like epoxide.
    • Osmium tetroxide is commonly used as a fixative and stain, which can interfere with subsequent staining.
    • Traditional hematoxylin and eosin (H&E) staining may yield suboptimal results on sections pre-treated with these materials.

    Purpose:

    • To describe a modified hematoxylin and eosin staining method for semi-thin sections.
    • To address challenges associated with staining sections after epoxide resin and osmium tetroxide removal.
    • To optimize contrast and detail in histological samples.

    Summary:

    • A novel protocol for H&E staining of semi-thin sections is detailed.
    • The method involves preliminary treatment with potassium permanganate and oxalic acid to enhance hematoxylin uptake.
    • Differentiation and iron chloride toning are performed after Harris hematoxylin staining, followed by a specific eosin mixture for counterstaining.

    Impact:

    • Provides a reliable method for clear visualization of cellular structures in prepared semi-thin sections.
    • Improves the quality of histological images obtained from challenging samples.
    • Facilitates accurate morphological analysis in research and diagnostics.

    Related Experiment Videos