Differential backfilling of interneuron populations based upon axon projections in a lobster abdominal ganglion

Intersegmental interneurons in the lobster second abdominal ganglion were differentially stained by simultaneously backfilling their axons in the anterior and posterior hemiconnectives with nickel and cobalt ions. When precipitated with rubeanic acid the nickel and cobalt formed different colors in the cell bodies depending upon the location of the axon(s) in the connectives. In the most completely stained preparations 55 ascending, 56 descending, and 25 bidirectional interneurons were observed. Soma diameters ranged from 15-120 micron. One of the somata was located in the connective. The differential staining technique has advantages over conventional backfilling techniques for examining the morphological relationships of populations of neurons having different axon projections.