The forensic laboratory evaluation of evidence in alleged rape

Serial vaginal fluid samples were obtained for laboratory analysis in 15 couples after voluntary intercourse. These samples were analyzed to determine the rate of decay of sperm and prostatic acid phosphatase as it pertains to alleged rape. An attempt was made to identify the male partner by laboratory examination of pubic hair combings and for foreign ABO antigens in vaginal fluid. If consideration is given to proper laboratory controls, examination for spermatozoa and prostatic acid phosphatase remains the primary test in alleged rape.