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Acid phosphatase released to the growth medium by Rhodotorula glutinis. Purification and some properties of the
Abstract:
The purification procedure of acid phosphatase released by the cells of Rhodotorula glutinis to the growth medium is elaborated, as a result of which a homogeneous preparation of the enzyme is obtained. The properties of the enzyme isolated from the medium with the enzyme purified from the cells are compared. The differences in molecular weight, carbohydrate content, thermal and pH-stability and sensitivity towards SH-group reagents are found. Possible reasons for these differences are discussed.