Immunochemical studies on Shigella dysenteriae type 10 bacterial polysaccharide

On graded hydrolysis and Smith degradation, the O-somatic polysaccharide isolated from Shigella dysenteriae type 10 bacteria yielded four oligosaccharides which were characterized by methylation studies. Immunochemical studies using constituent monosaccharides and the oligosaccharides indicated that the sugar grouping leads to 3)-ManNAc-(1 leads to 3)-Rha-(1 leads to 4)-GlcNAc-(1 leads to was the immunodominant part in the polysaccharide molecule, and that 2-acetamido-2-deoxy-D-mannose contributed maximally to the immunological specificity of the macromolecule.