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Updated: Aug 8, 2026

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corroles
Published on: March 18, 2015
The 5'-terminal ribonucleotide of an in vitro ColE1 transcript is not complementary to the DNA template
Abstract:
A set of overlapping promoters in ColE1 DNA is identified using DNA and RNA sequencing methods. These promoters initiate a divergent pattern of in vitro transcription from a region near the colicin E1 promoter. A comparison of the 5'-terminal RNA sequences of the in vitro transcripts with the DNA sequence of the template indicates that one transcript initiates with a ribonucleotide (pppG) which is not complementary to the DNA template (a thymidylate residue is present on the coding strand at the designated transcription start point). A structural and functional counterpart for this phenomenon is suggested to exist near the opposite end of the colicin E1 gene.
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Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
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Transcription
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