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[Use of DEAE-Sephadex A-50 microcarriers for reproduction of the mumps virus]
Abstract:
Reproduction of the vaccine L-3 strains of mumps virus was studied in cultures of continuous Vero cells and in primary cultures of Japanese quail embryos (JQE) growing on DEAE-Sephadex A-50 microcarriers. The Vero cell culture multiplies actively on the microcarrier surface giving more than a 20-fold increase in 8 days. Mumps virus showed a high reproductive capacity in Vero cell culture and in primary JQE cells. Mumps virus-infected Vero cells produce 5-6 pools of virus-containing material with a mean infectious titre 8.2-8.3 lg HAE50/ml. The primary JQE culture infected with mumps virus can yield 2-3 pools of virus-containing material. The intensity of mumps virus replication in the latter directly depends on the multiplicity of infection. Hemadsorption test could be performed in mumps virus-infected cell cultures on microcarriers.
Insights
This study demonstrates that both Vero cells and Japanese quail embryo cells grown on microcarriers effectively support mumps virus replication. These cell cultures provide a viable method for producing mumps virus for vaccine development.
Area of Science:
- Virology
- Cell Biology
- Vaccine Development
Background:
- Mumps virus vaccine production relies on efficient cell culture systems.
- Optimizing cell culture methods is crucial for high-yield vaccine strains.
Purpose of the Study:
- To evaluate the reproductive capacity of mumps virus vaccine L-3 strains in two distinct cell culture systems.
- To assess the suitability of Vero cells and Japanese quail embryo (JQE) cells grown on microcarriers for mumps virus propagation.
Main Methods:
- Culturing continuous Vero cells and primary JQE cells on DEAE-Sephadex A-50 microcarriers.
- Infecting cell cultures with mumps virus vaccine L-3 strains.
- Quantifying virus reproduction through infectious titer measurements (lg HAE50/ml) and hemadsorption tests.
Main Results:
- Vero cell cultures showed active multiplication on microcarriers, with over a 20-fold increase in 8 days.
- Mumps virus exhibited high reproductive capacity in both Vero cells and primary JQE cells.
- Vero cell cultures yielded 5-6 pools of virus with a mean infectious titer of 8.2-8.3 lg HAE50/ml.
- Primary JQE cultures yielded 2-3 pools, with replication intensity dependent on the multiplicity of infection.
Conclusions:
- Both Vero cells and primary JQE cells grown on microcarriers are effective systems for mumps virus reproduction.
- These microcarrier-based cell cultures offer a promising platform for the production of mumps virus for vaccine purposes.
- Hemadsorption testing is a feasible method for monitoring mumps virus infection in these cell cultures.