Related Experiment Video

Updated: Aug 18, 2026

Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition
08:01

Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition

Published on: December 22, 2011

Electron staining of the cell surface coat by osmium-low ferrocyanide

W F Neiss

    Histochemistry
    |January 1, 1984
    PubMed

    More Related Videos

    Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles11:16

    Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

    Published on: August 7, 2016

    Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy09:21

    Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

    Published on: July 20, 2019

    Related Concept Videos

    Fixation and Sectioning01:03

    Fixation and Sectioning

    Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
    The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
    Preparation of Samples for Electron Microscopy01:20

    Preparation of Samples for Electron Microscopy

    To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
    Immunogold Electron Microscopy01:20

    Immunogold Electron Microscopy

    Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
    Simple Staining Technique01:24

    Simple Staining Technique

    OverviewStaining techniques in microscopy enhance the visualization of microorganisms by increasing contrast and allowing the differentiation of cellular structures. Simple staining is one of the fundamental methods used to observe the basic morphological characteristics of microorganisms, including their size, shape, and arrangement. This method relies on the application of a single dye to stain the entire cell, producing a clear contrast between the cell and the background.FixationFixation is...
    Differential Staining Technique01:26

    Differential Staining Technique

    Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
    Special Staining Techniques01:13

    Special Staining Techniques

    Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...