Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bicarbonate uptake by basolateral membrane vesicles from rat jejunum.

A Faelli, M Tosco, M N Orsenigo

    Archives Internationales De Physiologie Et De Biochimie
    |December 1, 1983
    PubMed
    Summary

    This study isolated rat jejunal enterocyte basolateral membranes to investigate bicarbonate and L-glucose uptake. Researchers successfully measured bicarbonate flux under near-physiological conditions, demonstrating a viable method for future studies.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    "Reverse" dual-plane mammaplasty.

    Aesthetic plastic surgery·2006
    Same author

    Dermal apoptosis is not influenced by the severity of clinical conditions in burned patients.

    European review for medical and pharmacological sciences·2006
    Same author

    More than two different bacterial species isolated from blood of toxic epidermal necrolysis patients significantly increase mortality.

    Burns : journal of the International Society for Burn Injuries·2006
    Same author

    Endocannabinoids and beta-amyloid-induced neurotoxicity in vivo: effect of pharmacological elevation of endocannabinoid levels.

    Cellular and molecular life sciences : CMLS·2006
    Same author

    Foot angiolipomas: the third case of the literature.

    European review for medical and pharmacological sciences·2006
    Same author

    Toxic epidermal necrolysis in patients with malignancies.

    Annals of oncology : official journal of the European Society for Medical Oncology·2006

    Area of Science:

    • Cell Biology
    • Gastrointestinal Physiology
    • Membrane Transport

    Background:

    • Basolateral membranes of jejunal enterocytes play a crucial role in nutrient and ion absorption.
    • Understanding ion transport mechanisms is vital for comprehending intestinal function and disease.

    Purpose of the Study:

    • To isolate and characterize basolateral membrane vesicles from rat jejunal enterocytes.
    • To investigate the uptake kinetics of bicarbonate and L-glucose across these vesicles.
    • To establish a method for measuring bicarbonate flux under near-physiological conditions.

    Main Methods:

    • Isolation of basolateral membrane vesicles using self-orienting Percoll-gradient centrifugation.
    • Measurement of bicarbonate and L-glucose uptake via rapid filtration technique.

    Related Experiment Videos

  • Kinetic analysis of transport at pH 8.2 and 18°C.

    • Main Results:

    • Successfully obtained osmotically active basolateral membrane vesicles.
    • Bicarbonate uptake was observed to be rapid.
    • Inhibition studies using SITS and DIDS showed negligible effects on bicarbonate transport.

    Conclusions:

    • The study successfully developed a method to determine bicarbonate flux across rat jejunal enterocyte basolateral membrane vesicles.
    • The established technique allows for flux measurements under experimental conditions approximating physiological pH and temperature.
    • This methodology provides a foundation for further research into basolateral membrane transport mechanisms in the jejunum.