Selective extraction by 1-butanol of surface glycoprotein antigens from human melanoma cells

The butanol extraction method has previously been used to achieve selective release of tumor-specific transplantation antigens from mouse sarcoma cells. In this study we investigated the feasibility of this method for extracting four surface glycoprotein antigens (87K, 95-150K, HLA-DR, and HLA-A,B,C) from cultured human melanoma cells. Of the four antigens examined, only 95-150K and HLA-DR antigens could readily be detected in material extracted by 2%, 3%, or 5% butanol. The 3% butanol was found to be most effective in releasing these two antigens. Treatment of melanoma cells with less than or equal to 3% butanol did not decrease the viability of extracted cells as judged by either Trypan Blue dye exclusion or plating efficiency. Thus the noncytolytic butanol extraction method offers a promising approach to the isolation of certain glycoproteins such as 95-150K and HLA-DR from viable human melanoma cells for further purification and structural analysis.