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Updated: Jul 12, 2026

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation
Published on: October 19, 2009
The 3'-untranslated sequence of human skeletal muscle alpha-actin mRNA
Abstract:
A human actin cDNA clone pGF3 isolated from a fetal skeletal muscle cDNA library is described. The insert cDNA is homologous to skeletal muscle alpha-actin as judged by restriction mapping and nucleotide sequencing. The recombinant contains a substantial portion of the coding and the complete 3'-untranslated region. Comparison of the 3' ends of human and rat skeletal muscle and human cardiac alpha-actins reveals little homology between different types of actin genes in man but marked conservation of this region in the skeletal muscle actins of man and rat.
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