Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Folate deficiency and pancreatic acinar cell function.

M Elseweidy, M Singh

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
    |November 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effects of maternal labetalol on the newborn infant.

    Indian pediatrics·1992
    Same author

    Attenuation of murine acute lethal graft-versus-host disease by the administration of DL-alpha-difluoromethylornithine.

    Clinical immunology and immunopathology·1992
    Same author

    Prevention of hypoglycemia: a controlled evaluation of sugar fortified milk feeding in small-for-gestational age infants.

    Indian pediatrics·1992
    Same author

    Hypoplastic right ventricle with mild pulmonary stenosis in an adult.

    International journal of cardiology·1992
    Same author

    Enterotoxigenic drug resistant plasmids in animal isolates of Escherichia coli and their zoonotic importance.

    The Journal of tropical medicine and hygiene·1992
    Same author

    Gastric residuals in preterm babies.

    Journal of tropical pediatrics·1992
    Same journal

    Parasympathomimetic effect of aqueous humor in human eyes with and without chronic simple glaucoma.

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010
    Same journal

    Studies on mode of action of streptomycin.

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010
    Same journal

    Growth of influenza virus in eggs in the presence of bacterial contamination and streptomycin.

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010
    Same journal

    A convenient test animal for the detection of the leukocytosis-promoting factor of exudates.

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010
    Same journal

    Preparation of an inactivated tissue culture scrub typhus vaccine.

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010
    Same journal

    Studies on scrub typhus; preparation of formalinized vaccines from tissues of infected mice and rats.

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010
    See all related articles

    Folate deficiency impairs pancreatic acinar cell DNA synthesis, affecting pancreatic function. This suggests folate deficiency may contribute to pancreatic injury in malnutrition and alcoholism.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Nutritional Science

    Background:

    • Pancreatic acinar cells are crucial for digestive enzyme production.
    • Malnutrition and alcoholism are associated with pancreatic dysfunction.
    • The role of folate in pancreatic acinar cell function requires elucidation.

    Purpose of the Study:

    • To investigate the impact of folate deficiency on pancreatic acinar cell function in rats.
    • To determine if folate deficiency affects DNA synthesis, protein synthesis, or amylase secretion in pancreatic acinar cells.

    Main Methods:

    • Rats were fed folate-deficient, folate-sufficient, or regular diets.
    • Key parameters measured included body and pancreatic weight, DNA and RNA synthesis, protein content, and amylase secretion (basal and stimulated).

    Related Experiment Videos

  • Radioactive tracers ([methyl-14C]thymidine and [8-14C]adenine) were used to assess DNA and RNA synthesis.

    • Main Results:

    • Folate deficiency significantly impaired DNA synthesis, indicated by reduced [methyl-14C]thymidine incorporation into DNA.
    • No significant changes were observed in amylase content or secretion (basal or stimulated).
    • Folate repletion in deficient rats normalized the observed impairments.

    Conclusions:

    • Folate deficiency, not dietary antibiotics, impairs pancreatic acinar cell function, specifically DNA synthesis.
    • These findings suggest a potential role for folate deficiency in the pathogenesis of pancreatic injury associated with malnutrition and alcoholism.