[Induction of baboon herpes virus antigens in producing lymphoblastoid cells]

Treatment of lymphoid 594S/F9 cells with 12-o-tetradecanoylphorbol-13-acetate (TPA), cycloheximide (CH) has no effect on cell proliferation. A reversible blocking of the cellular DNA and RNA synthesis is observed. Repeated treatment with TPA, iododeoxyuridine (IDU) or sodium butyrate (SB) causes more pronounced changes in cell metabolism, which sharply decreases the salt sensitive DNA polymerase activity. Simultaneously the activity of the virus-induced ammonium sulphate resistant DNA polymerase increases. The combined effect of TPA and CH stimulates the synthesis of viral antigens in certain latently infected cells. The number of productively infected cells remains approximately at the same level for 96 h. IDU has a highest effect on viral antigens expression at repeated induction.