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Updated: Aug 16, 2026

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
Published on: August 25, 2018
[Determination of epidermal G2-chalone in epidermal-type tissues]
Abstract:
By means of monospecific immune serum, using the indirect method by Coons, the epidermal G2-chalone was revealed in the corneal epithelium, in the transitory epithelium of the urinary bladder, renal pelvis, as well as in stromal epithelial cells of the cortical substance and in thymic bodies, the facts that suggest epithelial nature of these tissues. In tracheal epithelium the method mentioned failed to reveal G2-chalone. Analysing localization of the epidermal G2-chalone in various tissues of the epidermal origin, it has been stated that in the non-cornified multistratified flat epithelium it is present in cellular cytoplasm of all layers, while in the cornified epithelium - it is predominantly detected in basal and scupular cells. A suggestion is made that distribution of the intratissue epidermal G2-chalone depends on the process of cornification. A possibility to use G2-chalone as a marker for tissues of the epidermal type is discussed.
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