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Related Experiment Videos

Transcription terminates at lambda tR1 in three clusters.

L F Lau, J W Roberts, R Wu

    Proceedings of the National Academy of Sciences of the United States of America
    |October 1, 1982
    PubMed
    Summary
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    Bacteriophage lambda transcription termination at tR1 involves three distinct sites. Rho factor sensitivity varies, with site III being most sensitive, and specific mutations affect termination site II.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Virology

    Background:

    • Bacteriophage lambda transcription relies on specific termination regions.
    • The rightward operon termination site, tR1, is crucial for lytic repressor cro gene expression.

    Purpose of the Study:

    • To precisely map transcription termination sites at bacteriophage lambda's tR1 region.
    • To investigate the roles of rho factor, L factor, and specific mutations in termination efficiency.

    Main Methods:

    • Nuclease S1 mapping was employed to determine transcript endpoints in vitro.
    • Analysis of termination site sensitivity to rho and L factors.
    • Examination of termination patterns in cin-lcnc-l mutant bacteriophages.

    Main Results:

    Related Experiment Videos

    • Transcription termination at tR1 occurs over a 60-nucleotide region with three distinct sites (I, II, III).
    • Site I termination is inhibited by L factor; sites II and III are not.
    • Rho factor sensitivity follows the order: III > II > I.
    • The cin-lcnc-l mutations abolish termination at site II, but not I or III.

    Conclusions:

    • Multiple termination sites exist at lambda tR1 with differential regulation by factors and mutations.
    • The cin-lcnc-l mutation's effect on site II explains its inability to rescue N function.
    • No direct correlation was found between RNA stem-loop structures and termination efficiency at these sites.