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[Methods of isolating immune cells--critical assessment, artifacts]
Allergie Und Immunologie
|January 1, 1982
Summary
This paper reviews common immune cell separation techniques, evaluating their purity, yield, and potential artifacts for researchers. It covers methods from density-gradient to cell sorting for effective immune cell isolation.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Context:
- Accurate immune cell separation is crucial for understanding immune responses and developing therapies.
- Various methods exist, each with specific advantages and limitations.
- Researchers require critical evaluations to select optimal isolation protocols.
Purpose:
- To provide a comprehensive overview of prevalent immune cell separation techniques.
- To critically assess the yield, purity, and potential artifacts associated with each method.
- To guide researchers in choosing the most suitable isolation strategy based on experimental needs.
Summary:
- Discusses density-gradient separation, cell electrophoresis, adherence, affinity chromatography, antisera/complement isolation, cell sorting, monolayer, and lectin-based methods.
- Evaluates techniques based on efficiency (yield) and precision (purity).
- Highlights potential experimental artifacts and procedural complexities for each method.
Impact:
- Facilitates informed selection of immune cell isolation techniques.
- Aids in optimizing experimental design for immunological studies.
- Contributes to reproducible and reliable research in immunology and related fields.