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Related Experiment Videos

Setting up and running a microscope-based flow cytometer.

S E Petersen

    Cytometry
    |January 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    A novel, noncommercial flow cytometry system was developed using a fluorescence microscope. This high-resolution instrument offers precise cellular DNA and microsphere analysis with minimal variation.

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    Area of Science:

    • Biomedical Engineering
    • Analytical Chemistry
    • Cell Biology

    Background:

    • Flow cytometry systems often require specialized engineering support.
    • Conventional fluorescence microscopes can be adapted for flow analysis.
    • Novel flow principles offer potential for improved instrumentation.

    Purpose of the Study:

    • To develop a noncommercial, high-resolution flow cytometry system.
    • To demonstrate the feasibility of building such a system without dedicated engineering staff.
    • To evaluate the system's precision in analyzing cellular DNA and microspheres.

    Main Methods:

    • Adaptation of a conventional fluorescence microscope.
    • Implementation of a novel flow principle (Steen and Lindmo, 1979).

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  • Introduction of specific modifications and additions to the base system.

    • Main Results:

    • Achieved high resolution and ease of adjustment/control.
    • Obtained coefficients of variation as low as 0.8% for cellular DNA.
    • Obtained coefficients of variation as low as 0.9% for fluorescent microspheres.

    Conclusions:

    • A noncommercial flow cytometry system can be successfully constructed and operated in a standard laboratory.
    • The developed system provides high-resolution analysis comparable to commercial instruments.
    • This approach offers a cost-effective and accessible solution for flow cytometry applications.