[Is there anionic ATPase in the nuclei of animal cells?]

The nuclei of the rat liver, heart, thymus and of the mouse liver isolated in sucrose gradient reveal ATPase sensitive to bicarbonate, sulfite, azide and thiocyanate. The admixture of mitochondria and submitochondrial particles in the nuclear preparation was found negligible, which could not contribute to the anion ATPase in the nuclei. This was demonstrated by the calculation and by the introducing of mitochondria into the nuclear preparations.