Demonstration of the existence, and partial characterization, of a cell-surface beta-hexosaminidase from rat...

Rat splenocytes are shown to exhibit cell-surface located beta-N-acetylglucosaminidase and beta-galactosidase activities. Preincubation experiments, solubilization experiments and chemical cross-linking experiments show that these enzymatic activities are indeed cell-surface localized. The solubilization and partial purification of the beta-N-acetylglucosaminidase activity is reported. Kinetic studies of the partially purified material with a variety of competitive inhibitors at several pH values suggest that at physiological pH the cell surface beta-N-acetylglucosaminidase may function as a carbohydrate binding protein rather than as a glycosidase.