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Transferability of antibiotic resistance determinants in strains of Staphylococcus aureus belonging to different
Abstract:
A total of 107 donor strains of Staphylococcus aureus isolated from clinical material, with a high incidence of multiresistant strains belonging predominantly to phage group III, were tested for transmission of determinants of resistance to 6 antibiotics using mixed cultures of donor strains and the recipient Staphylococcus aureus strain 5849-fur-r, rif-r. The capability of strains to transfer resistance markers to the recipient was found to depend neither on phage group nor phage type to which the donor strain belonged, but strains possessing multiple resistance to antibiotics effectuated transfers at comparatively higher frequencies.
Insights
Multiresistant Staphylococcus aureus strains efficiently transfer antibiotic resistance markers. Resistance transfer capability was independent of phage type but higher in strains with multiple resistances.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Staphylococcus aureus is a significant human pathogen.
- The emergence of antibiotic-resistant strains poses a global health threat.
- Understanding the mechanisms of resistance transfer is crucial for effective treatment strategies.
Purpose of the Study:
- To investigate the transmission of antibiotic resistance determinants in Staphylococcus aureus.
- To determine factors influencing the frequency of resistance transfer between strains.
Main Methods:
- Utilized 107 clinical isolates of Staphylococcus aureus as donor strains.
- Employed mixed culture experiments with a recipient strain (Staphylococcus aureus 5849-fur-r, rif-r).
- Assessed the transfer of resistance to six different antibiotics.
Main Results:
- The ability of strains to transfer antibiotic resistance markers was not dependent on phage group or type.
- Strains exhibiting multiple resistances demonstrated higher frequencies of resistance marker transfer.
- A high incidence of multiresistant strains, predominantly from phage group III, was observed.
Conclusions:
- Phage typing is not a reliable predictor of antibiotic resistance transfer capability in Staphylococcus aureus.
- Multiple antibiotic resistance in donor strains correlates with increased frequency of resistance transfer.
- These findings highlight the potential for rapid dissemination of antibiotic resistance within Staphylococcus aureus populations.