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High-performance liquid chromatography for assaying NAD glycohydrolase from Neurospora crassa conidia
Analytical Biochemistry
|June 1, 1983
Abstract:
A rapid and sensitive high-performance liquid chromatographic technique was developed to determinate NAD glycohydrolase (EC 3.2.2.5.) activity from Neurospora crassa conidia. The separation of the assay substrate and products was achieved by isocratic reverse-phase chromatography and the peaks were detected by the absorbance at 259 nm. Quantities of NAD+ and nicotinamide as small as 10 pmol could be measured.