Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cl--stimulated adenosine triphosphatase: existence, location and function.

G A Gerencser, S H Lee

    The Journal of Experimental Biology
    |September 1, 1983
    PubMed
    Summary

    This study explores chloride transport mechanisms, investigating the potential role of anion-stimulated ATPases in moving chloride against its gradient across cell membranes. Further research is needed to confirm this direct transport function.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Sodium-Sulfate Symport by Aplysia californica Gut.

    Zoological science·2008
    Same author

    Cl(-)-ATPases: biological active transporters.

    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology·2002
    Same author

    Transport energetics of the Na+ pump in Aplysia californica gut.

    Canadian journal of physiology and pharmacology·2001
    Same author

    Effect of Laticauda semifasciata (sea snake) venom on chloride transport across the frog skin.

    Toxicon : official journal of the International Society on Toxinology·2001
    Same author

    Phosphorylation of chloride-ATPase reconstituted from Aplysia gut.

    The Journal of experimental zoology·2001
    Same author

    The Aplysia californica Cl- pump is a P-type ATPase: evidence through inhibition studies.

    Canadian journal of physiology and pharmacology·2001

    Area of Science:

    • Cellular physiology
    • Membrane transport
    • Biochemistry

    Background:

    • Chloride transport across plasma membranes typically involves sodium-coupled symport, anion-coupled antiport, or coupling to primary ion transport.
    • Direct evidence for primary chloride transport is lacking, despite observations of cellular anion-stimulated ATPases.
    • Anion-stimulated ATPases are known mitochondrial components and are present in various subcellular locations, including plasma membranes.

    Purpose of the Study:

    • To investigate the potential involvement of plasma membrane-located anion-stimulated ATPases in primary chloride transport.
    • To explore the possibility of ATPases facilitating chloride movement against its electrochemical gradient.

    Main Methods:

    • Indirect evidence was gathered through parallel studies correlating anion-stimulated ATPase activity with chloride fluxes in the same tissue.
    • Analysis focused on cellular and subcellular locations of anion-stimulated ATPase activity.

    Main Results:

    • Anion-stimulated ATPases are a ubiquitous property of animal cells, with significant activity observed in plasma membranes.
    • Indirect evidence suggests a potential role for ATPase in the net movement of chloride against its electrochemical gradient.

    Conclusions:

    • While anion-stimulated ATPases are present in plasma membranes, their direct role in primary chloride transport requires further substantiation.
    • Additional studies are necessary to confirm the direct transport function of Cl--stimulated ATPases in the plasma membrane.

    Related Experiment Videos