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Spectinomycin resistant gonococci.

C A Ison, K Littleton, K P Shannon

    British Medical Journal (Clinical Research Ed.)
    |December 17, 1983
    PubMed
    Summary
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    Spectinomycin-resistant gonococci isolates in London exhibited beta-lactamase production and specific plasmid profiles. Resistance transfer varied, with some isolates linked to spectinomycin therapy.

    Area of Science:

    • Microbiology
    • Genetics
    • Public Health

    Background:

    • Neisseria gonorrhoeae causes sexually transmitted infections.
    • Antimicrobial resistance in gonococci is a growing global health concern.
    • Spectinomycin is an important antibiotic for gonorrhea treatment.

    Purpose of the Study:

    • To characterize clinical isolates of spectinomycin-resistant Neisseria gonorrhoeae.
    • To investigate the genetic and phenotypic properties of these resistant strains.
    • To explore potential mechanisms of resistance and their clinical associations.

    Main Methods:

    • Analysis of 10 clinical isolates of spectinomycin-resistant gonococci.
    • Plasmid profiling and characterization.
    • Testing for beta-lactamase production and aminoglycoside modifying enzymes.

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  • Transformation experiments to assess resistance transfer.

    • Main Results:

    • All 10 isolates produced beta-lactamase and contained plasmids (2.6, 4.4, 24.5 Mdaltons).
    • Proline was required for growth; no aminoglycoside modifying enzymes were detected.
    • Spectinomycin resistance transfer occurred at a lower frequency than streptomycin resistance.
    • Isolates from St Mary's Hospital were linked to prior spectinomycin therapy, unlike those from St Thomas's Hospital.

    Conclusions:

    • Spectinomycin resistance in these gonococcal isolates is associated with beta-lactamase production and specific plasmid carriage.
    • Mechanisms other than aminoglycoside modifying enzymes are involved in spectinomycin resistance.
    • The findings suggest a potential link between spectinomycin use and the emergence of resistant strains in specific clinical settings.