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Bacteriophage lambda preconnectors. Purification and structure.

J Kochan, J L Carrascosa, H Murialdo

    Journal of Molecular Biology
    |April 15, 1984
    PubMed
    Summary
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    Bacteriophage lambda prohead assembly involves a gpB polymer called a preconnector. This study partially purified and structurally characterized these preconnectors, revealing a 12-fold symmetric, donut-like structure crucial for morphogenesis.

    Area of Science:

    • Molecular Biology
    • Structural Biology
    • Virology

    Background:

    • Bacteriophage lambda prohead morphogenesis is regulated by phage genes (B, C, Nu3, E) and E. coli genes (groEL, groES).
    • Previous studies identified a biologically active gpB polymer, termed a preconnector, accumulating in lambda C-E- mutant infections as a prohead assembly intermediate.

    Purpose of the Study:

    • To partially purify and structurally characterize the biologically active bacteriophage lambda preconnector.
    • To elucidate the three-dimensional structure of the preconnector and its implications for prohead assembly.

    Main Methods:

    • Partial purification of biologically active preconnectors from infected bacterial cells.
    • Electron microscopy and optical filtration of preconnector arrays to determine structural symmetry and morphology.

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  • Construction of a three-dimensional model based on structural data.

    • Main Results:

    • Biologically active preconnectors were partially purified.
    • Electron microscopy revealed donut-like structures with radial symmetry.
    • Optical filtration demonstrated 12-fold rotational symmetry; side views resembled asymmetrical dumbbells.
    • A three-dimensional model of the preconnector was constructed.

    Conclusions:

    • The characterized preconnector structure provides insights into its role in bacteriophage lambda prohead morphogenesis.
    • The findings contribute to understanding the mechanisms of viral DNA packaging and head assembly.