Related Experiment Video
Updated: Aug 23, 2026

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
Published on: July 22, 2011
[Electrophoretic typing of Escherichia coli esterases in septicemia]
Abstract:
Esterases produced by 175 strains of Escherichia coli isolated from 34 patients with septicaemia were subjected to electrophoresis in acrylamide-agarose gel. Forty-four electrophoretic types were identified, and the septicaemias were divided into two groups according to whether or not all the strains isolated in a given patient were of the same electrophoretic type. In the first group (21 patients) the presence of one single electrophoretic type demonstrated that the strains isolated from haemocultures and those isolated from focal specimens were identical and could be held responsible for infection in different foci. In the second group (13 patients) the presence of different electrophoretic types in the same patient either discard an relationship between strain from haemoculture and strain from focal specimen, or demonstrated that different strains could coexist in a focus of infection, or revealed successive and overlapping infections. These results not only provide precise information on the nature of bacteria responsible for infections, but also have interesting pathophysiological and epidemiological implications.
Related Concept Videos
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
Effects of EDTA on End-Point Detection Methods
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...
Electrophoresis: Overview
There...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Bacterial Gastroenteritis

