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[Changes in spontaneous platelet aggregation induced by the ambient temperature].

A Taccola, G B Gotti, G P Zelaschi

    Minerva Medica
    |April 21, 1984
    PubMed
    Summary

    Temperature significantly impacts platelet function tests. Sedimenting samples at 37°C, not room temperature, provides accurate results for spontaneous platelet aggregation (P.A.) and cardiovascular pathology assessment.

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    Area of Science:

    • Hematology
    • Cardiovascular Pathology
    • Biomedical Research

    Context:

    • Functional platelet modifications, particularly spontaneous platelet aggregation (P.A.), hold diagnostic significance in cardiovascular pathology.
    • Accurate assessment requires methods mimicking real haemorheological conditions.
    • Previous studies have not fully addressed temperature's influence on sample preparation for platelet function testing.

    Purpose:

    • To evaluate the impact of sample temperature variations during plasma separation on spontaneous platelet aggregation (P.A.) and beta-thromboglobulin (beta-TG) excretion.
    • To determine optimal conditions for reliable platelet function testing in cardiovascular disease assessment.

    Summary:

    • Research compared platelet aggregation and beta-TG excretion using plasma sedimented at 37°C versus room temperature in 731 subjects.

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  • Significant variations in spontaneous P.A. were observed, with lower temperatures favoring increased beta-TG excretion.
  • Sedimentation at 37°C yielded plasma suitable for accurate platelet testing, while room temperature processing may artificially activate platelets.

    • Impact:

    • Findings suggest that sample sedimentation at 37°C is crucial for reliable platelet function tests, including P.A.
    • Room temperature plasma separation may lead to overestimated platelet activation and aggregation.
    • Establishes a new normal range for spontaneous P.A. within 3% aggregability.
    • Highlights the need to standardize pre-analytical conditions for platelet function assays to ensure diagnostic accuracy in cardiovascular pathology.