Related Experiment Video

Updated: Aug 11, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
15:22

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

A simplified procedure for synthesizing large quantities of highly purified uridine

L Lang, S Kornfeld

    Analytical Biochemistry
    |July 1, 1984
    PubMed

    Abstract:

    A simplified procedure for the synthesis of [beta-32P]UDP-N-acetylglucosamine is described. This novel method utilizes commercially available enzymes, chemical acetylation, and preparative thin-layer chromatography and can be used to synthesize millicurie quantities of the sugar nucleotide at an extremely high specific activity and purity.

    More Related Videos

    Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
    11:08

    Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

    Published on: February 5, 2018

    Regioselective O-Glycosylation of Nucleosides via the Temporary 2',3'-Diol Protection by a Boronic Ester for the Synthesis of Disaccharide Nucleosides
    08:46

    Regioselective O-Glycosylation of Nucleosides via the Temporary 2',3'-Diol Protection by a Boronic Ester for the Synthesis of Disaccharide Nucleosides

    Published on: July 26, 2018

    Related Experiment Videos

    Last Updated: Aug 11, 2026

    Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
    15:22

    Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

    Published on: April 3, 2014

    Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
    11:08

    Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

    Published on: February 5, 2018

    Regioselective O-Glycosylation of Nucleosides via the Temporary 2',3'-Diol Protection by a Boronic Ester for the Synthesis of Disaccharide Nucleosides
    08:46

    Regioselective O-Glycosylation of Nucleosides via the Temporary 2',3'-Diol Protection by a Boronic Ester for the Synthesis of Disaccharide Nucleosides

    Published on: July 26, 2018

    Related Concept Videos

    Preparation of 1° Amines: Gabriel Synthesis01:28

    Preparation of 1° Amines: Gabriel Synthesis

    Direct alkylation is not a suitable method for synthesizing amines because it produces polyalkylated products. Gabriel synthesis is the most preferred method to exclusively make primary amines. The method uses phthalimide, which contains a protected form of nitrogen that participates in alkylation only once to predominantly give primary amines.
    Strong bases like NaOH or KOH deprotonate the phthalimide to form the corresponding anion, which acts as a nucleophile. Further, the anion attacks an...
    Biosynthesis of Polysaccharides01:26

    Biosynthesis of Polysaccharides

    Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...

    Articles linked to this work by shared authors, journal, and citation graph.

    [Progress in basic research on hydrogel-based drug delivery systems for the treatment of peri-implantitis].

    Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2026

    [Mechanism of reactive oxygen species regulation in bone metabolism and its significance in bone-related oral diseases].

    Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2025

    Ashwagandha: Is It Safe? Part 1: A Regulatory Review.

    Phytotherapy research : PTR·2025

    Evaluations of Endosseous Dental Implant Success Rates, Adverse Events, and Associated Risk Factors: A Retrospective Study.

    The International journal of oral & maxillofacial implants·2025

    The health economic burden of dengue in Germany: a retrospective analysis of statutory health insurance claims data.

    Infectious diseases now·2025

    I know why the caged bird sings: Distress tolerant individuals show greater resting state connectivity between ventromedial prefrontal cortex and right amygdala as a function of higher vagal tone.

    International journal of psychophysiology : official journal of the International Organization of Psychophysiology·2023

    Fe-Co bimetallic nanozyme-amplified lateral-flow immunoassay for the sensitive detection of porcine rotavirus.

    Analytical biochemistry·2026

    Integrated sustainability evaluation of electrochemical quantification of flibanserin and caffeine in various matrices.

    Analytical biochemistry·2026

    A NanoBiT-based high-throughput assay system for monitoring osteoclast fusion.

    Analytical biochemistry·2026

    Integrating artificial intelligence with spectrophotometry: A novel python framework for automated green assessment and pharmaceutical analysis.

    Analytical biochemistry·2026

    HF-MMLLE using natural deep eutectic solvents for determination of potential lung cancer biomarkers in urine.

    Analytical biochemistry·2026

    Best practices for isothermal titration calorimetry of protein-DNA interactions.

    Analytical biochemistry·2026

    A Proline-Based 'Redox-Release' Platform for Programmed Access to Diverse Sulfur(VI) and Fluoroalkyl Radicals.

    Research square·2026

    An RNase Toxin Hijacks Elongation Factor-Tu to Cleave the Ribosomal Sarcin-Ricin Loop.

    bioRxiv : the preprint server for biology·2026

    OPA1-dependent mitochondrial remodeling coordinates TCR signaling and metabolic adaptation during iNKT cell differentiation.

    bioRxiv : the preprint server for biology·2026

    An intersectional expression platform for gene complementation using RNA-fragment end joining (REJ).

    bioRxiv : the preprint server for biology·2026

    SEME enables programmable transcript reconstruction through synthetic microexon incorporation.

    bioRxiv : the preprint server for biology·2026

    Eliminating manganese sensitivity and boosting citric acid production in Aspergillus niger through transporters manipulation.

    Microbial cell factories·2026
    See all related articles
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies
    Jove
    Visualize
    Contact Us