Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Proteases in human tumors.

P Burtin, G Chavanel, M C Fondaneche

    Bulletin Du Cancer
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Researchers investigated proteases in human colorectal tumors, finding plasminogen is abundant. This suggests plasminogen activation may facilitate tumor invasion and metastasis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Is intra-arterial hepatic chemotherapy painful?

    Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2020
    Same author

    Cancer of the pancreas: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up.

    Annals of oncology : official journal of the European Society for Medical Oncology·2015
    Same author

    Post-first-line FOLFOX chemotherapy for grade 3 neuroendocrine carcinoma.

    Endocrine-related cancer·2015
    Same author

    Peritoneal carcinomatosis from solid pseudopapillary neoplasm (Frantz's tumour) of the pancreas treated with HIPEC.

    Anticancer research·2012
    Same author

    Results of systematic second-look surgery plus HIPEC in asymptomatic patients presenting a high risk of developing colorectal peritoneal carcinomatosis.

    Annals of surgery·2011
    Same author

    Toxicity and antioxidant activity in vitro and in vivo of two Fucus vesiculosus extracts.

    Journal of agricultural and food chemistry·2008
    Same journal

    [Update: Evolution in the management of metastatic urothelial carcinomas].

    Bulletin du cancer·2026
    Same journal

    [ONCONNECTE À L'EMPLOI, feedback after three years of a program to prepare for returning to work after cancer in Franche-Comté].

    Bulletin du cancer·2026
    Same journal

    [Off-label use of venetoclax in myeloma].

    Bulletin du cancer·2026
    Same journal

    [Cemiplimab - Adjuvant treatment for the cutaneous squamous cell carcinomas with high risk of relapse, operated and treated by radiotherapy].

    Bulletin du cancer·2026
    Same journal

    Real-world outcomes and management of endometrial cancer in France from 2016 to 2021 (MOONBEAM study).

    Bulletin du cancer·2026
    Same journal

    [Cardiotoxicity in children and adolescents with acute leukemia: Recommendations from the Leukemia Committee of the French Society of Childhood Cancer (SFCE)].

    Bulletin du cancer·2026
    See all related articles

    Area of Science:

    • Oncology
    • Biochemistry
    • Molecular Biology

    Background:

    • Proteases play a crucial role in tumor progression, including invasion and metastasis.
    • The plasmin system, involving plasminogen and its activators/inhibitors, is implicated in extracellular matrix degradation.
    • Understanding protease involvement in colorectal cancer is vital for developing targeted therapies.

    Purpose of the Study:

    • To investigate the presence and potential role of proteases, particularly the plasmin system and Cathepsin B, in colorectal adenocarcinomas.
    • To determine the localization of plasminogen and active plasmin within tumor tissues.
    • To explore the relationship between protease expression and tumor invasiveness.

    Main Methods:

    • Immunohistological techniques, including immunofluorescence and immunoperoxidase staining.

    Related Experiment Videos

  • Histochemical methods to detect enzyme activity.
  • Analysis of 30 colorectal adenocarcinoma samples.

    • Main Results:

    • An antigen reacting with anti-plasminogen serum was detected in all 30 colorectal adenocarcinomas studied.
    • Active plasmin was not detected using histochemical techniques, suggesting plasminogen is the predominant form.
    • High concentrations of plasmin inhibitors (alpha 2 antiplasmin, alpha 2 macroglobulin) were found in the peritumoral stroma.
    • Cathepsin B was identified in many colorectal tumors, but its role remains preliminary.

    Conclusions:

    • Plasminogen is significantly present in colorectal tumors and may be converted to active plasmin at tumor cell surfaces and invasive fronts.
    • Plasmin activity likely contributes to basement membrane degradation, facilitating tumor cell invasion and metastasis.
    • Inhibitors in the stroma rapidly neutralize plasmin, suggesting localized and transient activity.
    • Further research is needed to elucidate the precise role of Cathepsin B in colorectal tumor invasiveness.