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A high-pressure-liquid-chromatography method for the determination of corticosterone in rat plasma
Acta Physiologica Et Pharmacologica Latinoamericana : Organo De La Asociacion Latinoamericana De Ciencias Fisiologicas Y De La Asociacion Latinoamericana De Farmacologia
|January 1, 1984
Abstract:
A high-pressure-liquid-chromatography (HPLC), isotope dilution method for the determination of corticosterone in rat plasma is reported. Main characteristics of the technique are: efficient separation of the steroid from 18-HO-DOC and 11-deoxycortisol; two easy pre-purification steps consisting of an acetone extraction-deproteinization and fractionation on Sephadex LH-20; a sensitivity of 5 ng per injection and the possibility of determining 25 ng corticosterone in approximately one milliliter rat plasma.