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Multiple forms of renin substrate in human plasma.
Clinica Chimica Acta; International Journal of Clinical Chemistry
|February 15, 1978
Summary
Human plasma contains multiple forms of renin substrate (angiotensinogen), identified using chromatography and isoelectric focusing. These forms show consistent distribution across various physiological states and treatments.
Area of Science:
- Biochemistry
- Endocrinology
- Proteomics
Background:
- Renin substrate, also known as angiotensinogen, is a key component of the renin-angiotensin system.
- Previous studies suggested heterogeneity in renin substrate, but detailed characterization was limited.
Purpose of the Study:
- To investigate the existence and characteristics of multiple renin substrate forms in human plasma.
- To analyze the distribution of these forms under different physiological conditions.
Main Methods:
- DEAE-cellulose chromatography with descending pH gradient elution.
- Isoelectric focusing using ampholytes in a pH 3.5-5 range.
Main Results:
- Two major and several minor renin substrate forms were resolved by DEAE-cellulose chromatography, eluting predominantly near pH 4.8 and 4.4.
- Isoelectric focusing revealed up to eight distinct renin substrate forms, with four major forms identified in plasma from a woman on estrogen therapy.
- The elution and focusing patterns of these forms remained relatively consistent across normal individuals and those with diabetes, pregnancy, or estrogen therapy.
Conclusions:
- Human plasma renin substrate exhibits significant molecular heterogeneity.
- The distribution of renin substrate forms is largely conserved across different physiological states, suggesting a stable underlying structure or regulatory mechanism.