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Collagenase in human cornea: immunologic localization.

J M Gordon, E A Bauer, A Z Eisen

Archives of Ophthalmology (Chicago, Ill. : 1960)

|February 1, 1980

Summary

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Human corneal collagenase is present in ulcerating corneas but not in nonulcerating ones. This enzyme, found in the corneal stroma, may play a role in corneal disease pathogenesis.

Area of Science:

Ophthalmology
Biochemistry
Cell Biology

Background:

Collagenase is an enzyme crucial for tissue remodeling.
Its presence and role in human corneal pathology are not fully understood.

Purpose of the Study:

To localize collagenase within the human cornea.
To investigate the association of corneal collagenase with ulcerative conditions.

Main Methods:

Immunocytochemical techniques using specific antiserum to human skin collagenase.
In vivo analysis of ulcerating and nonulcerating corneas.
Organ culture of corneal tissue.

Main Results:

Collagenase was detected in vivo specifically in ulcerating human corneas.

Related Experiment Videos

No immunologic evidence of collagenase was found in nonulcerating corneas.

The enzyme was localized exclusively to the stromal portion of the cornea.

Conclusions:

Corneal stromal cells are the likely source of collagenase.
The presence of collagenase correlates with corneal ulceration.
Organ culture can model conditions relevant to corneal disease.