Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hexose uptake enhancing factor released from Rous sarcoma cells.

D A Lawrence, P Jullien

    Journal of Cellular Physiology
    |February 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Correction: TRAF2 is a biologically important necroptosis suppressor.

    Cell death and differentiation·2025
    Same author

    Newborn adipokines and early childhood growth.

    Pediatric obesity·2018
    Same author

    Impact of parental obesity on neonatal markers of inflammation and immune response.

    International journal of obesity (2005)·2016
    Same author

    Passenger mutations and aberrant gene expression in congenic tissue plasminogen activator-deficient mouse strains.

    Journal of thrombosis and haemostasis : JTH·2016
    Same author

    Grating coupled SPR microarray analysis of proteins and cells in blood from mice with breast cancer.

    The Analyst·2015
    Same author

    Mechanisms Underlying Astrocyte Endfeet Swelling in Stroke.

    Acta neurochirurgica. Supplement·2015
    Same journal

    Mt1-Ca<sup>2+</sup>-Mitochondrial Metabolic Axis Maintains Temporomandibular Joint Condylar Cartilage Homeostasis Under Low Oxygen and Hypoxic Condition.

    Journal of cellular physiology·2026
    Same journal

    Correction to "IRE1α/NOX4 Signaling Pathway Mediates Ros-Dependent Activation of Hepatic Stellate Cells in NaAsO<sub>2</sub>-Induced Liver Fibrosis".

    Journal of cellular physiology·2026
    Same journal

    Lipocalin-2 Restores Mitochondrial and Antioxidant Adaptation in a Strain-Specific Manner During LPS Induced Sepsis.

    Journal of cellular physiology·2026
    Same journal

    Dysregulation of Non-Muscle Myosin IIA Assembly and Phosphorylation in S100A4 Null Mouse Lens.

    Journal of cellular physiology·2026
    Same journal

    Corrigendum to "A Probiotic Combination of Limosilactobacillus reuteri and Clostridium butyricum Ameliorates 5-Fluorouracil-Induced Intestinal Mucositis in Rats by Strengthening Barrier Integrity and Modulating Immuno-Microbial Homeostasis".

    Journal of cellular physiology·2026
    Same journal

    Sexually Dimorphic Regulation of MiR-29a/c-3p in Human Endothelial Cells: Cell Functions and Transcriptome.

    Journal of cellular physiology·2026
    See all related articles

    Conditioned media from transformed chicken cells contain a factor that enhances glucose uptake in normal cells. This factor, approximately 20,000 daltons, is heat-sensitive and may relate to glucose transport proteins.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Virology

    Background:

    • Rous sarcoma virus (RSV) transformation alters cellular functions.
    • Transformed cells release factors influencing normal cells.
    • Glucose uptake is a key metabolic indicator of cell state.

    Purpose of the Study:

    • To identify and characterize the factor in RSV-transformed chicken embryo fibroblast conditioned media that stimulates 2-deoxyglucose uptake in normal fibroblasts.
    • To determine the properties of this stimulatory factor.

    Main Methods:

    • Preparation of conditioned media from RSV-transformed and normal chicken embryo fibroblasts.
    • Assay of 2-deoxyglucose uptake in normal fibroblasts treated with conditioned media.
    • Enzymatic treatment (trypsin) and gel filtration to characterize the factor.

    Related Experiment Videos

  • Assessment of protease and plasminogen activator activities.

    • Main Results:

    • Conditioned media from RSV-transformed cells significantly increased 2-deoxyglucose uptake in normal fibroblasts.
    • The active factor is destroyed by trypsin, indicating it is proteinaceous.
    • The factor's activity is independent of protease and plasminogen activator activities.
    • Gel filtration estimated the factor's molecular weight at approximately 20,000 daltons.
    • A similar factor, albeit in lower amounts, is shed by non-transformed fibroblasts.

    Conclusions:

    • A heat-sensitive protein factor, around 20,000 daltons, present in conditioned media from RSV-transformed chicken cells stimulates glucose uptake in normal cells.
    • This factor may be related to glucose binding and transport proteins.
    • The findings suggest a potential role for secreted factors in mediating metabolic changes during viral transformation.