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Related Experiment Videos

Complement receptors: specific detection by molecular complexes.

J Cossman, J C Glorioso, R Adler

    Journal of Immunological Methods
    |January 1, 1978
    PubMed
    Summary
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    Bovine serum albumin-anti-BSA immune complexes activate human complement via the alternative pathway. This activates mononuclear cells, offering a sensitive method for complement detection.

    Area of Science:

    • Immunology
    • Complement System Biology

    Background:

    • Immune complexes are crucial in immune responses.
    • The alternative complement pathway plays a role in innate immunity.
    • Complement receptors on mononuclear cells mediate immune cell interactions.

    Purpose of the Study:

    • To investigate the activation of human complement by BSA-anti-BSA immune complexes.
    • To explore the binding of complement component C3 to human peripheral blood mononuclear cells.
    • To develop and evaluate a sensitive assay for detecting complement activation.

    Main Methods:

    • Formation of immune complexes using BSA-anti-BSA (IgG F(ab')2).
    • Activation of the alternative complement pathway.
    • Detection of C3 binding to mononuclear cells using fluoresceinated BSA (BSA-FITC) or anti-C3 antisera.

    Related Experiment Videos

  • Comparison with the IgMEAC rosette technique.

    • Main Results:

    • BSA-anti-BSA immune complexes activate the alternative complement pathway.
    • C3 from activated complement binds to mononuclear cells expressing complement receptors.
    • The developed assay demonstrates higher sensitivity than the IgMEAC rosette technique.
    • The assay results show good correlation with the established rosette method.

    Conclusions:

    • The alternative complement pathway is activated by BSA-anti-BSA immune complexes.
    • Mononuclear cell complement receptors bind C3-complexes, enabling visualization.
    • This assay provides a sensitive and reliable method for detecting complement activation.