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Related Experiment Videos

The structure of poliovirus replicative form.

G R Larsen, A J Dorner, T J Harris

    Nucleic Acids Research
    |March 25, 1980
    PubMed
    Summary

    Investigating polio replicative form (RF) structure revealed the 3' end sequence of the minus strand complements the 5' end of polio RNA. This suggests a genetically coded 5' terminal U and a protruding poly(A) tail on the plus strand.

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    Area of Science:

    • Virology
    • Molecular Biology
    • RNA Structure

    Background:

    • The replicative form (RF) of poliovirus is a double-stranded RNA intermediate crucial for viral replication.
    • Understanding the precise structure of RF is essential for elucidating viral replication mechanisms.

    Purpose of the Study:

    • To elucidate the complete structure of the poliovirus replicative form (RF).
    • To investigate the 3' terminal sequence of the minus strand and the poly(A) tail of the plus strand within the RF.

    Main Methods:

    • Utilized 3' end labeling techniques to analyze the RNA structure.
    • Employed polynucleotide phosphorylase for enzymatic degradation and structural analysis.

    Main Results:

    • Established a complete composite structure of the poliovirus RF.
    • Demonstrated that the 3' terminal sequence of the minus strand is a precise complement to the 5' end of poliovirus RNA.
    • Confirmed a genetically coded poly(A) tract on the plus strand, with an additional single-stranded poly(A) tail protruding from the double-stranded RNA.

    Conclusions:

    • The findings suggest that the 5' terminal uracil (U) of poliovirus RNA is genetically coded.
    • The structure of the RF indicates a complex polyadenylation mechanism involving both coded and protruding poly(A) sequences.

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