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Related Experiment Videos

Collagen-bound collagenase.

A Pardo, H Soto, I Montfort

    Connective Tissue Research
    |January 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Collagenase enzyme bound to collagen is a physiological reality, not an artifact. This bound collagenase is associated with extracellular collagen fibers in living animals.

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Histology

    Background:

    • Collagenase is an enzyme that degrades collagen.
    • The interaction between collagen and collagenase has been studied, but its physiological relevance is debated.
    • Standard extraction procedures may introduce artifacts.

    Purpose of the Study:

    • To confirm the presence of collagenase bound to collagen.
    • To investigate whether collagen-bound collagenase is a physiological entity or an artifact of extraction.
    • To characterize the properties of collagen-bound collagenase.

    Main Methods:

    • Collagen extraction and purification from animal and human sources.
    • Polyacrylamide gel electrophoresis (PAGE) at different concentrations and pH.

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  • SDS-PAGE analysis.
  • TCA-ethanol purification cycles and pepsin digestion.
  • Monospecific antibody production and inhibition assays.

    • Main Results:

    • Electrophoresis separated collagen from a collagen-binding protein with collagenolytic activity.
    • Removal of the binding protein eliminated collagen's "spontaneous" degradation.
    • Antibodies against the binding protein inhibited collagenase activity.
    • The binding protein was identified as collagenase.

    Conclusions:

    • Collagen-bound collagenase is a physiological reality, not an artifact of extraction.
    • This enzyme is likely associated with extracellular collagen fibers in vivo.
    • The findings suggest a direct role for collagenase in collagen remodeling within the extracellular matrix.